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    • Main Office
      Burkert South Africa (PTY) Ltd
      Visitors address:

      233 Albert Amon Road
      Millennium Business Park
      Meadowdale / Germiston

      Mailing address:

      P.O. Box 26260
      East Rand
      1462
      Phone +27 11 574 6000
      Fax +27 11 454 1477
      E-Mail fnyrf.mn@ohexreg.pbz
      Website http://www.buerkert.co.za/
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      32 Grays-Inn Crescent
      Westville

      Phone +27 31 266 1600
      Fax +27 31 266 1495
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      Unit 26, Northgate Business Park
      Northgate Estate

      Phone +27 21 510 7137
      Fax +27 21 510 7135
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Liquid chromatography

"Chromatography" is an umbrella term covering physical methods in which substances are separated via distribution between a stationary and mobile phase.

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HPLC (High-Performance Liquid Chromatography) is a separating process in which a liquid sample is transported through a stationary phase under high pressure. Depending on the nature of the interaction between the phases and the sample, we distinguish between adsorption chromatography, partition chromatography, ion-exchange chromatography, sizeexclusion chromatography and affinity chromatography. The processes of adsorption chromatography and partition chromatography are the chief processes used in HPLC. HPLC uses partition particles with grain sizes of a few µm; it thus achieves high numbers of plates or trays, but at the same time, requires a high counter-pressure to be overcome during transport of the mobile phase through the thin separation column (of a few millimeters diameter). All parts must be interconnected without any dead volume wherever possible and must be pressure-stable (up to 300 bar).

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